Guangzhao Mao
Professor, Chemical Engineering and Materials Science
313-577-3804 (Phone)
Biography
| 2007 to present | Professor | Department of Chemical Engineering and Materials Science, WSU. | |
| 2001 to 2007 | Associate Professor | Department of Chemical Engineering and Materials Science, WSU. | |
| 1995 to 2001 | Assistant Professor | Department of Chemical Engineering and Materials Science, WSU. | |
| 2010 to present | Co-director | Nano Incubator Program, WSU. | |
| 2001 to present | Adjunct Professor | Department of Biomedical Engineering, WSU. | |
| 1997 to present | Director | Graduate Certificate Program in Polymer Engineering, WSU. | |
| 1994 to 1995 | Postdoctoral Associate | University of Minnesota (Advisor: Prof. Michael D. Ward). | |
| 1989 to 1994 | Ph.D. Student | Department of Chemical Engineering and Materials Science, University of Minnesota. | |
Education
Ph.D., Chemical Engineering, University of Minnesota, 1994
(Advisors: Prof. Matthew Tirrell and Prof. H. Ted Davis)
Postdoctoral Associate, Materials Science, University of Minnesota, 1995
(Advisor: Prof. Michael D. Ward)
B.Sc., Chemistry, Nanjing University, P. R. China, 1988
Research Interests
- Nanoscale Science and Engineering
- Colloid and Surface Science
- Polymer Science and Engineering
Research Statement and other Highlights:
The Mao group conducts research to understand the role of molecular interactions in molecular self-assembly. The laboratory is equipped with a variety of interfacial and nanomaterial characterization methods including AFM and X-ray reflectivity.
Engineering hybrid nanomaterials:
Surface-bound nanoparticle arrays are components in single-electron transistors, surface plasmon and surface-enhanced Raman scattering substrates, and ultrasensitive electrochemical sensors. The Mao group uses nanoparticles as seeds to nucleate nanocrystals. The research tests the hypothesis of shape-restrictive non-epitaxial nucleation and growth from a nanoparticle surface. This project provides a fundamental understanding of the crystallization and seed-mediated nucleation processes used widely in industry for material separation and purification. The project addresses an issue at the heart of crystal engineering, how much area is necessary for heterogeneous nucleation. This work is notable because it not only creates new hybrid nanomaterials but also contributes to the understanding of the impact of diminishing seed size on heterogeneous nucleation and crystallization.
Engineering polymeric nanoparticles and thin films for gene delivery:
The promise of gene therapy to treat a variety of genetic and acquired diseases has fueled research on effective gene delivery systems. Currently the field is dominated by "black-box" strategies that test reporter gene expression levels of various formulations. In contrast, the Mao group conducts research on how and when plasmid DNA is dissociated from its complexes with polycations, a question that is critical to overall gene delivery efficiency. The long-term goal is to develop a toolkit of bio-responsive polycation carriers to enable the release of therapeutic nucleic acids at the right place and time. The research tests the hypothesis that the timing and extent of polyplex disassembly are a critical limiting step to overall gene delivery efficiency. The research reveals the intra- and inter-polyplex structural heterogeneity and the role of molecular interactions among the intermediates.
Research Projects
- Self-Assembly in Nanoconfinement (Research Nugget)
- Using Nanoparticles as Seeds for Molecular Crystallization (Research Nugget)
- Stimuli-Controlled Dissociation of DNA from Polymer-Coupled Assemblies (Research Nugget)
- Bio-Recognition Force Measurements for Phage-Based Sensors (Research Nugget)
- Nanotechnology-Based Approaches for Understanding Pathogenesis (Research Nugget)
Publications
- Gary, D.; Lee, LH.; Sharma, R.; Lee, J.-S. Kim, Y.; Cui, Z. Y.; Jia, Di. Bowman, V.; Chipman, P. Wan, L.; Zou, Y.; Mao, G.; Park, K.; Herbert, B.-S.; Konieczny, S.; Won, Y.-Y. The Influence of Nano-Carrier Architecture on In Vitro siRNA Delivery Performance and In Vivo Biodistribution: Polyplexes vs. Micelleplexes, ACS Nano, accepted, 2011.
- Wang, R.; Li, L.; Arachchige, I.; Ganguly, S.; Brock, S. L.; Mao, G. Nanoparticles Change the Ordering Pattern of n-Carboxylic Acids into Nanorods on HOPG, ACS Nano, 4, 6687-6696, 2010. PDF document
- Zou, Y.; Wan, L.; Mao, G. Atomic Force Microscopy Studies of DNA Release in Gene Delivery Dynamics in Microscopy Book Series - Volume # 4: “Microscopy: Science, Technology, Applications and Education”, A. Mendez-Vilas and J. D. Alvarez Eds.; Formatex Research Center, Dec. 2010. pp. 489-499. PDF document
- Wan, L.; Li, L.; Mao, G. Nanospiral Formation by Droplet Drying: One Molecule at a Time, Nanoscale Res. Lett. DOI 10.1007/s11671-010-9793-9, 2010. PDF document
- Mao, G. et al. Particle-rod Nano Structures and Method of Forming Same by Spin Coating, U. S. Patent Trademark Office patent 7,709,054, 05/04/2010.
- Handa, H.; Gurczynski, S.; Jackson, M. P.; Mao, G. Immobilization and Molecular Interactions between Bacteriophage and Lipopolysaccharide Bilayers, Langmuir, 26, 12095-12103, 2010. PDF document
- Blacklock, J.; Torsten, S.; Handa, H.; You, Y.-z.; Oupicky, D.; Mao, G.; Mohwald, H. Cross-linked Bioreducible Layer-by-layer Films for Increased Cell Adhesion and Transgene Expression, J. Phys. Chem. B, 114, 5283-5291, 2010. PDF document
- Blacklock, J.; Mao, G.; Oupicky, D.; Mohwald, H. DNA Release Dynamics from Bioreducible Layer-by-layer Films, Langmuir, 26, 8597–8605, 2010. PDF document
- Wan, L.; You, Y.; Zou, Y.; Oupicky, D.; Mao, G. DNA Release from Bioreducible Poly(amido amine) Polyplexes, J. Phys. Chem. B, 113, 13735-13741, 2009. PDF document
- Blacklock, J.; You, Y.-Z.; Mao, G.; Oupicky, D. Gene Delivery in vitro and in vivo from Bioreducible Multilayered Polyelectrolyte Films of Plasmid DNA, Biomaterials, 30, 939-950, 2009. PDF document
- Han, H. J.; Kannan, R. M.; Wang, S.; Romera, R.; Mao, G. Dendrimer-based Diagnostic Nanodevice for Improved Detection of Inflammatory Markers in the Amniotic Fluid, Adv. Funct. Mater. 19, 1-13, 2009. PDF document
- Kakade, S.; Manickam, D. S.; Handa, H.; Mao, G.; Oupický, D. Cellular Uptake and Transfection Activity of Layer-by-Layer Plasmid DNA/Poly(ethylenimine) Films Deposited on PLGA Microparticles, Int. J. Pharm., 365, 44-52, 2009. PDF document
- Wang, R.; Dong, W.; Mao, G. Template Synthesis of Low Dimensional Organic and Ceramic Hybrid Materials (invited) in Encyclopedia of Nanoceramics and Their Based Nanodevices, T.-Y. Tseng, H. S. Nalwa, Eds.; American Scientific Publishers, Chap. 14, 2009.
- Khdair, A.; Handa, H.; Mao, G.; Panyam, J. Nanoparticle-mediated Combination Chemotherapy and Photodynamic Therapy Overcomes Tumor Drug Resistance In vitro, Euro. J. Pharm. Biopharm. 71, 214-222, 2009. PDF document
- Mao, G.; Newaz, G.; Wei, C. ENATBIO: a National Conference on Emerging Nanoscience in Technology and Biomedicine, Nanomedicine: Nanotechnology, Biology, and Medicine, 4, 267-272, 2008. PDF document
- Wan, L.; Manickam, D. S.; Oupicky, D.; Mao, G. DNA Release Dynamics from Reducible Polyplexes by AFM, Langmuir, 24, 12474-12482, 2008. PDF document
- Khdair, A,; Gerard, B.; Handa, H.; Mao, G.; Shekhar, M. P. V.; Panyam, J.; Surfactant-Polymer Nanoparticles Enhance the Effectiveness of Anticancer Photodynamic Therapy, Mol. Pharmaceutics, 5, 795-807, 2008. PDF document
- Handa, H.; Gurczynski, S.; Jackson, M. P.; Auner, G.; Walker, J.; Mao, G. Recognition of Salmonella Typhimurium by Immobilized Phage P22 Monolayers, Surface Science, 602, 1392-1400, 2008. PDF document
- Wan, L.; Harender, B.; You, Y.-Z.; Oupicky, D.; Mao, G. Temperature-Reversible Ultrathin Films of N-Isopropylacrylamide Terpolymer Adsorbed at the Solid-Liquid Interface, Langmuir, 23, 12159-12166, 2007. PDF document
- Salakhutdinov, I.; VandeVord, P.; Palyvoda, O.; Matthew, H.; Tatagiri, G.; Handa, H.; Mao, G.; Auner, G. W.; Newaz, G. Fibronectin Adsorption to Nanopatterned Silicon Surfaces, Journal of Nanomaterials, doi:10.1155/2008/543170, 2008. PDF document
- Shahani, K.; Jonnalagadda, S.; Handa, H.; Mao, G.; Panyam, J. Phospholipid Nanoparticles: Process Optimization Using Fractional Factorial Design and Atomic Force Microscopy, J. Biomed. Nanotechnol. 3(4), 394-400, 2007. Abstract
- You, Y.-Z.; Zhou, Q.-H.; Manickam, D. S.; Wan, L.; Mao, G.; Oupický, D. Dually Responsive Multiblock Copolymers via RAFT Polymerization: Synthesis of Temperature- and Redox-responsive Copolymers of PNIPAM and PDMAEMA, Macromolecules, 40, 8617-8624, 2007. PDF document
- Wang R.; Arachchige, I. U.; Brock, S. L.; Mao, G. Nanoparticles as Seeds for Organic Crystallization in ACS Symposium Series: Nanoparticles: Synthesis, Stabilization, Passivation and Functionalization, Chapter 25, 358-368, 2008.
- Chen, D.; Handa, H.; Wan, L.; Mao, G. Surface Morphological Evolution of Ultrathin P4VP Films and Generation of Ordered Pattern on Graphite, Macromol. Rapid Comm., 28, 1619-1623, 2007. PDF document
- Chavanpatil, M. D.; Khdair, A.; Patil, Y.; Handa, H.; Mao, G.; Panyam, J. Polymer-Surfactant Nanoparticles for Sustained Release of Water-Soluble Drugs, J. Pharm. Sci., 96, 3379-3389, 2007. PDF document
- Chavanpatil, M. D.; Handa, H.; Mao, G.; Panyam, J. Incorporation of Phospholipids Enhances Cellular Uptake and Retention of Surfactant-Polymer Nanoparticles, J. Biomed. Nanotechnol., 3, 291-296 , 2007. Abstract
- Blacklock, J.; Handa, H.; Manickam, D. S.; Mao, G.; Mukhopadhyay, A.; Oupicky, D. Disassembly of Layer-by-Layer Films of Plasmid DNA and Reducible TAT Polypeptide, Biomaterials, 28, 117-124, 2007. PDF document
- Haynie, D. T.; Rudra, J. S.; Palath, N.; Zhang, L.; Handa, H.; Mao, G. Polypeptide Multilayer Nanofilms for Cell and Tissue Engineering, PMSE Prepr. 97, 236, 2007.
- Subramnian, V.; Sands, M. B.; Mao, G. Thermal Characterization of the Interaction of Silanes with a Dihydroxy Vulcanized Fluoroelastomer, Rubber Chem. Technology, 79 765-782, 2006. Abstract
- Arumugam, P.; Shinozaki, S. S.; Wang, R.; Mao, G.; Brock, S. L. From Ribbons to Nanodot Arrays: Reduction of Nickel Arsenate to Nickel Arsenide, Chem. Comm., 1121-1123, 2006. PDF document
- Mao, G.; Liang, X. Resolving the Thickness and Micromechanical Properties of Lipid Bilayers and Vesicles Using AFM, an invited review chapter in Force Microscopy in Biology and Medicine, B. P. Jena and J. K. H. Hörber Eds.; Wiley-LISS: Hoboken, NJ 2006; Chap. 11. PDF document
- Dong, J.; Wang, A.; Ng, K. Y. S.; Mao, G. Self-Assembly of Octadecyltrichlorosilane Monolayers on Silicon-Based Substrates by Chemical Vapor Deposition, Thin Solid Films 515, 2116-2122, 2006. PDF document
- Dong, W.; Wang, R.; Mao, G.; Möhwald, H. Self-Assembled Molecular Patterns of Fatty Acid in the Presence of Metal Ions, Soft Matter 2, 686-692, 2006. PDF document
- Zhao, Y.; Mao, G.; Wang, J. Self-assembled Nanostructures for Antireflection Optical Coatings, Proc. SPIE, 5642, 355, 2005 (doi:10.1117/12.577122).
- Dong, W.; Liu, S.; Wan, L.; Kurth, D. G.; Mao, G.; Möhwald, H. Controlled Permeability in Polyelectrolyte Films via Solvent Treatment, Chem. Mater., 17, 4992-4995, 2005. PDF document
- Bishta, H. S.; Wan, L.; Mao, G.; Oupicky, D. pH-Controlled Association of PEG-Containing Terpolymers of N-Isopropylacrylamide and 1-Vinylimidazole, Polymer, 46, 7945-7952, 2005. PDF document
- Dong, J.; Mao, G. Polystyrene Nanorod Formation in C12E5 Hemimicelle Thin Film Templates, Colloid Polym. Sci. 284, 340–345, 2005. PDF document
- Zhao, Y.; Wang, J.; Mao, G. Colloidal Subwavelength Nanostructures for Antireflection Optical Coatings, Optics Lett. 30, 1885-1887, 2005. PDF document
- Mao, G.; Chen, D.; Handa, H.; Dong, W.; Kurth, D. G.; Möhwald, H. The Deposition and Aggregation of Aspirin Molecules on a Phospholipid Bilayer Pattern, Langmuir, 21, 578-585, 2005. PDF document
- Manickam, D. S.; Bisht, H. S.; Wan, L.; Mao, G.; Oupicky, D. Influence of TAT-Peptide Polymerization on Properties and Transfection Activity of TAT/DNA Polyplexes, J. Controlled Release, 102, 293-306, 2005. PDF document
- Chen, D.; Wang, R.; Arachchige, I.; Mao, G.; Brock, S. L. Particle-Rod Hybrids: Growth of Arachidic Acid Molecular Rods from Capped Cadmium Selenide Nanoparticles, J. Am. Chem. Soc., 126, 16290-16291, 2004. PDF document
- Liang, X.; Mao, G.; Ng, K. Y. S. Effect of Chain Lengths of PEO-PPO-PEO on Small Unilamellar Liposome Morphology and Stability: An AFM Investigation, J. Colloid Interface Sci., 285, 360-372, 2005. PDF document
- Liang, X.; Mao, G.; Ng, K. Y. S. Mechanical Properties and Stability Measurement of Cholesterol-Containing Liposome on Mica by Atomic Force Microscopy, J. Colloid Interface Sci., 278, 53-62, 2004. PDF document
- Dong, J.; Mao, G.; Hill, R. M. Nanoscale Aggregate Structures of Trisiloxane Surfactants at the Solid-Liquid Interface, Langmuir, 20, 2695-2700, 2004. PDF document
- Mao, G.; Dong, W.; Kurth, D.; Möhwald, H. Synthesis of Copper Sulfide Nanorod Arrays on Molecular Templates, Nano Lett., 4, 249-252, 2004. PDF document
- Mao, G.; Liang X.; Ng, K. Y. S. Direct Force Measurement of Liposomes by Atomic Force Microscopy in The Dekker Encyclopedia of Nanoscience and Nanotechnology, J. A. Schwarz, C. I. Contescu, and K. Putyera, Eds.; Marcel Dekker, Inc.: New York, 923-932, 2004. PDF document
- Ngankam, A.; Mao, G.; Van Tassel, P. Fibronectin Adsorption onto Polyelectrolyte Multilayer Films, Langmuir, 20, 3362-3370, 2004. PDF document
- Liang, X.; Mao, G.; Ng, K. Y. S. Probing Unilamellar EggPC Vesicles by Atomic Force Microscopy, Colloids and Surfaces B, 34, 41-51, 2004. PDF document
- Dong, J.; Mao, G.; Hill, R. M. AFM Study of Trisiloxane Surfactant Aggregate Structures at the Solid/Liquid Interface in Mesoscale Phenomena in Fluid Systems, ACS Symposium Series 861, Chap. 1, 2-16, 2003. PDF document
- Wang, A., Jiang, L.; Mao, G., Liu, Y. Direct Force Measurement of Silicone- and Hydrocarbon-Based ABA Triblock Surfactants in Alcoholic Media by Atomic Force Microscopy, J. Colloid Interface Sci., 256, 331-340, 2002. PDF document
- Wang, A., Jiang, L.; Mao, G., Liu, Y. Direct Force Measurement of Comb Silicone Surfactants in Alcoholic Media by Atomic Force Microscopy, J. Colloid Interface Sci., 242, 337-345, 2001. PDF document
- Dong, J., Mao, G. Direct study of C12E5 Aggregation on Mica by AFM Imaging and Force Measurements, Langmuir, 16, 6641-6647, 2000. PDF document
- Li, B., Avrustsky, I., Zhao, Y., Mao, G. Statistical Study of Two-Dimensional Colloidal Crystals Based on Microscopic Images and Optical Diffraction, Colloids and Surfaces, 174, 113-119, 2000. PDF document
- Li, H.; Mao, G.; Ng, K. Y. S. AFM Study of Template Growth of Cadmium Sulfide Nanoparticle Using Pure and Mixed Arachidate Films, Thin Solid Films, 358, 62-72, 2000. PDF document
- Tao, J., Mao, G., Daehne, L. Asymmetrical Molecular Aggregation in Spherulitic Dye Films, J. Am. Chem. Soc., 121, 3475-3485, 1999. PDF document
- Li, M., Wang, A., Mao, G., Dähne, L. Surface-Directed Adsorption in the Epitaxy Growth of Streptocyanine Dye Crystals, J. Phys. Chem., 103, 11161-11168, 1999. PDF document
- Wu, B., Mao, G., Ng, S. K. Y. Stepwise Adsorption of a Long Trichlorosilane and a Short Aminosilane, Colloids and Surfaces A, 162, 203-213, 1999. PDF document
- Dähne, L., Tao, J., Mao, G. Surface Morphological Study of J-Aggregate Thin Films by Atomic Force Microscopy, Langmuir, 14, 565-568, 1998. PDF document
- Mao, G., Lobo, L., Scaringe, R.; Ward, M. Nanoscale Visualization of Crystal Habit Modification by Atomic Force Microscopy, Chem. Mater., 9, 773-783, 1997. PDF document
- Tirrell, M.; Davis, H. T.; Mao, G. Self-Assembled Polymers: Photopolymerizable Bolaform Amphiphiles in the Polymeric Materials Encyclopedia: Synthesis, Properties and Applications, J. C. Salamone ed. CRC Press, 10, 7600-7611, 1996. PDF document
- Mao, G.; Tsao, Y.; Tirrell, M.; Davis, H.; Hessel, V.; Ringsdorf, H. Interactions, Structure and Stability of Photoreactive Bolaform Amphiphile Multilayers Langmuir, 11, 942-952.1995. PDF document
- Mao, G.; Tsao, Y.; Tirrell, M.; Davis, H.; Hessel, V.; van Esch, J.; Ringsdorf, H. Monolayers of Bolaform Amphiphiles: Influence of Alkyl Chain Length and Counterions Langmuir, 10, 4174-4184, 1994. PDF document
- Mao, G.; Tsao, Y.; Tirrell, M.; Davis, H. T.; Hessel, V.; Ringsdorf, H. Self-Assembly of Photopolymerizable Bolaform Amphiphile Mono- and Multilayers Langmuir, 9, 3461-3470, 1993. PDF document
- Ananthapadmanabhan, K. P.; Mao, G. -z.; Goddard, E. D.; Tirrell, M. Surface Forces Measurements on a Cationic Polymer in the Presence of an Anionic Surfactant Colloids and Surfaces, 61, 167-174, 1991. PDF document
Awards and Honors
2009-10 2009 2008 2002 | Experienced GRFP Resource Person Symposium Organizer Excellence in Teaching Award Fulbright Scholar | National Science Foundation, USA. ACS Nationation Meeting. College of Engineering, WSU. Department of State, USA. | |
| 2002 | Visiting Professor | Max Planck Institute of Colloids and Interfaces, Germany. | |
| 1997 | Faculty CAREER Award | National Science Foundation, USA. | |
Accomplishments
- March 7, 2012
Guangzhao Mao, will host a Go-Girls group in May as part of her commitment to provide a nanomaterials module to a NIH grant. The group will visit the nanomaterials lab for an hour and will complete a survey using the College's computer lab.
In the News
- Guangzhao Mao was featured on WWJ-TV's website for hosting former participants in the Gaining Options - Girls Investigate Real Life (GO-GIRL) program at the College of Engineering. At the event, called GO-GIRL Material Girls event, Mao and Assistant Professor Eranda Nikolla gave tours of the chemical engineering nanomaterials laboratory and the energy materials laboratory.
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